MCF2L Knockout cell line (HCT 116)

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MCF2L Knockout cell line (HCT 116) Order

MCF2L Knockout cell line (HCT 116)
Catalog#
YKO-HS08869
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MCF2L Knockout cell line (HCT 116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MCF2L Knockout cell line (HCT 116)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
MCF2L
Gene ID
23263
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 4 (based on transcript MCF2L-232)
Validation: PCR+Sanger Sequencing
MCF2L Knockout cell line (HCT 116) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCF2L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCF2L gene knockout MCF2L Knockout cell line (HCT 116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MCF2L Knockout cell line (HCT 116)
MCF2L Knockout cell line (HCT 116)
Catalog#
YKO-HS08869
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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